Diagnostic Value of the Methylation of Multiple Gene Promoters in Serum in Hepatitis B Virus-Related Hepatocellular Carcinoma

Table 1

List of primers and probes for MethyLight.

Gene	Primer and TaqMan probe sequences (5 → 3)
RASSF1A	Forward primer: GCGTTGAAGTCGGGGTTC
	Reverse primer: CCCGTACTTCGCTAACTTTAAACG
	TaqMan probe: FAM-FAM-ACAAACGCGAACCGAACGAAACCA-BHQ1
APC	Forward primer: AGTGCGGGTCGGGAAGC
	Reverse primer: AACCACATATCGATCACGTACG
	TaqMan probe: FAM-AAAACGCCCTAATCCGCATCCAACG-BHQ1
BVES	Forward primer: GGACGGAGTGGGCGATATC
	Reverse primer: CCTCGAACCGCGCAAA
	TaqMan probe: FAM-CCTACGTACAACCGAACG-MGB
TIMP3	Forward primer: GCGTCGGAGGTTAAGGTTGTT
	Reverse primer: CTCTCCAAAATTACCGTACGCG
	TaqMan probe: FAM-AACTCGCTCGCCCGCCGAA-BHQ1
GSTP1	Forward primer: CGTCGTGATTTAGTATTGGGGC
	Reverse primer: CTAATAACGAAAACTACGACGACGAAA
	TaqMan probe: FAM-ATAAGGTTCGGAGGTCGCGAGGTTTTCGT-BHQ1
HOXA9	Forward primer: AATAAATTTTATCGTAGAGCGGTAC
	Reverse primer: CATATAACAACTTAATAACACCGAA
	TaqMan probe: FAM-GCGCCCCCATTAACCGTACGCGT-BHQ1
ACTB	Forward primer: TGGTGATGGAGGAGGTTTAGTAAGT
	Reverse primer: AACCAATAAAACCTACTCCTCCCTTAA
	TaqMan probe: FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ1