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Disease Markers
Volume 2017, Article ID 4209257, 8 pages
https://doi.org/10.1155/2017/4209257
Research Article

Association of GCLM -588C/T and GCLC -129T/C Promoter Polymorphisms of Genes Coding the Subunits of Glutamate Cysteine Ligase with Ischemic Heart Disease Development in Kazakhstan Population

1Institute of General Genetics and Cytology, Al-Farabi St. 93, Almaty, Kazakhstan
2Russian Medical University, Torekulovst 71, Almaty, Kazakhstan
3Leonard Davis School of Gerontology, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089, USA

Correspondence should be addressed to Liliya Skvortsova; ur.relbmar@555_ailil

Received 16 February 2017; Revised 31 May 2017; Accepted 8 June 2017; Published 5 July 2017

Academic Editor: Mariann Harangi

Copyright © 2017 Liliya Skvortsova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Glutamate cysteine ligase (GCL) is a rate-limiting enzyme in synthesis of glutathione. Evidence suggests that genetic variations in the promoter region of genes coding a catalytic subunit (GCLC -129T/C) and a modifier subunit (GCLM -588C/T) of GCL have a functional impact on their transcriptional activity and were associated with various disorders. Hence, we hypothesize whether these two polymorphic variants of GCLM and GCLC genes are associated with the risk of ischemic heart disease (IHD) development in the population of Kazakhstan. Methods. We evaluated 360 patients with IHD and 341 control subjects. Allele frequencies of studied promoters’ polymorphisms were detected by PCR-RFLP analysis. Multiple logistic regression analysis was applied to assess the risk for different genotypes obtained. Results. The presence of -588T allele in GCLM and -129T allele in GCLC gene genotypes was associated with an increased risk of IHD (GCLM -588T: , ; GCLC -129T: , ) for general ethnically mixed group. Analysis of each ethnical groups separately showed the higher risk tendency for Kazakhs as for GCLM -588T (; ) and as for GCLC -129T (, ). For Russians, statistically differences for two polymorphisms were not observed. Conclusion. The two promoter polymorphisms of GCLM (-588C/T) and GCLC (-128T/C) are associated with an increased risk of IHD in Kazakhstan population.