miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP⁺-Intoxicated SH-SY5Y Cells

Figure 2

Expression level of GSK3β in MPP⁺-intoxicated SH-SY5Y cells. qRT-PCR and western blot were performed to assess the mRNA and protein expression of GSK3β in SH-SY5Y cells treated with different concentrations (0.25, 0.5, 1, and 2 mM) of MPP⁺ for 24 h (a and c) or incubated with 1 mM MPP⁺ for different duration (6 h, 12 h, 24 h, and 48 h) (b and d). versus control group.

(a)

(b)

(c)

(d)