miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP⁺-Intoxicated SH-SY5Y Cells

Figure 4

miR-135b directly targeted GSK3β. (a) The miR-135b binding sites in the 3′UTR of GSK3β. (b) Luciferase reporter assays were carried out in SH-SY5Y cells cotransfected with pmirGLO-GSK3β-3′-UTR-WT or pmirGLO-GSK3β-3′-UTR-MUT along with miR-135b or miR-NC. (c) qRT-PCR analysis of GSK3β mRNA expression in SH-SY5Y cells transfected with miR-135b mimic or inhibitor. (d and e) Western blot analysis of GSK3β in SH-SY5Y cells with miR-135b mimic or inhibitor transfection. versus control group.

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