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Disease Markers
Volume 2017 (2017), Article ID 5806236, 5 pages
Research Article

Genotyping of IL-4 −590 (C>T) Gene in Iraqi Asthma Patients

Department of Biology, College of Education for Pure Science-Ibn Al-Haytham, University of Baghdad, Baghdad, Iraq

Correspondence should be addressed to Ihsan A. Hussein

Received 1 December 2016; Revised 12 February 2017; Accepted 21 February 2017; Published 12 March 2017

Academic Editor: Hubertus Himmerich

Copyright © 2017 Ihsan A. Hussein and Saddam H. Jaber. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study is the first investigation in Iraq dealing with genotyping of IL-4 −590 (C>T) gene, especially in Iraqi patients with asthma. We studied forty-eight blood samples collected from patients with asthma and compared with age-matched 25 healthy individuals as controls. The polymorphism results of IL-4 −590 (C>T) gene by using amplification refractory mutation system (ARMS-PCR) showed the presence of C and T alleles and three genotypes (CC, CT, and TT). Interestingly the frequency of C allele and CC genotype was higher in patients with asthma in comparison with the same allele and genotype in control (P 1 × 10−6). This increase was associated with an increased risk factor of asthma (odds ratio [OR] 9.21; 95% confidence interval [CI] 3.58–23.71). Genotypes analysis by using Hardy-Weinberg distribution showed no significant differences between patients with asthma and healthy subjects. In conclusion, the increasing risk of asthma was associated with C allele and the CC genotype and these are revealed as etiological fraction with risk by having this disease, while the T allele percentage ratio in controls was higher when it is compared with asthma patients suggesting that these alleles have a protective effect (preventive fraction).