Research Article
Genotyping of IL-4 −590 (C>T) Gene in Iraqi Asthma Patients
Table 3
PCR conditions for genotyping of IL-4 gene position −590 (C>T).
| Steps | Temperature (°C) and cycles | Time (sec) |
| Denature template | 96 | 60 | First initial denaturation | 95 | 10 cycles | 15 | First annealing | 65 | 50 | First extension | 72 | 40 | Second initial denaturation | 95 | 20 cycles | 50 | Second annealing | 59 | 50 | Second extension | 72 | 50 | Final extension | 72 | 7 min | Incubation | 4 | 5 min |
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