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Disease Markers
Volume 2017 (2017), Article ID 6198526, 16 pages
https://doi.org/10.1155/2017/6198526
Research Article

Role of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 (Lys45Glu), MMP-7 (-181A/G), and MMP-12 (-82A/G) Variants and Plasma MMP Levels on Obesity-Related Phenotypes and Microvascular Reactivity in a Tunisian Population

1Unité de recherche UR12ES06, Physiologie de l’Exercice et Physiopathologie: de l’Intégré au Moléculaire “Biologie, Médecine et Santé,” Faculté de Médecine de Sousse, Université de Sousse, Sousse, Tunisia
2Faculté des Sciences de Bizerte, Université de Carthage, Bizerte, Tunisia
3Institut des Sciences Infirmières, Sousse, Tunisia
4District Medical du Centre, CNAM, Sousse, Tunisia
5Laboratory for Vascular Translational Science (LVTS), INSERM U1148, CHU X. Bichat, 46 rue Henri Huchard, 75877 Paris Cedex 18, France
6Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia

Correspondence should be addressed to Soumaya Boumiza

Received 2 July 2017; Revised 4 October 2017; Accepted 19 October 2017; Published 26 November 2017

Academic Editor: Michele Malaguarnera

Copyright © 2017 Soumaya Boumiza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. The impact of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 Lys45Glu (A/G), MMP-7 -181A/G, and MMP-12 -82A/G variants and plasma MMP levels on obesity and microvascular reactivity in Tunisians. Methods. Our population included 202 nonobese and 168 obese subjects. Anthropometric, biochemical, and microvascular parameters were determined according to standard protocols. PCR-RFLP and ELISA were used to determine the genetic variants and levels of MMPs, respectively. Results. The MMP-3 45Glu (G) allele associates with higher anthropometric values and MMP-3 levels compared to AA genotype carriers (BMI (kg/m2): 30 ± 0.51 versus 27.33 ± 0.8, ; MMP-3 levels: 7.45 (4.77–11.91) versus 5.21 (3.60–10.21) ng/ml, ). The MMP-12 -82G allele was also associated with higher BMI values when compared to subjects carrying the AA genotype (31.41 ± 0.85 versus 28.76 ± 0.43, ). Individuals carrying the MMP-3 45G or MMP-12 -82G variants were also associated with a higher risk for severe forms of obesity (MMP-3: OR = 1.9, ; MMP-12: OR = 2.63, ). Similarly, the MMP-7 -181G allele was associated with a higher MMP-7 level and an increased risk for morbid obesity when compared to AA genotype carriers (0.32 (0.31–0.60) versus 0.18 (0.17–0.24) ng/ml, ; OR = 1.67, , resp.). Conclusion. MMP-3, MMP-7, and MMP-12 polymorphisms associate with obesity risk and its severity.