Noncoding Centromeric RNA Expression Impairs Chromosome Stability in Human and Murine Stem Cells

Figure 2

Live cell imaging on 17α-expressing H2B-mCherry-HT1080 cells. The pIneo2 controls did not show any mis-segregation ((a), top, left to right). Expression of 17α satellite in HT1080 induced mis-segregation events ((a), middle and bottom, left to right). Time lapse images were taken every 15-minute interval, and the image prior to mitosis was set at time zero. There were several interesting phenotypic effects discovered in 17α-expressing HT1080 cells, stars ((b), A–D), binucleated cells (E–H), and polylobed nuclei (I–L).

(a)

(b)