Research Article
Noncoding Centromeric RNA Expression Impairs Chromosome Stability in Human and Murine Stem Cells
Table 1
The effects of noncoding centromeric RNAs on anaphase abnormalities.
| Expression vectors and
cell types | Anaphase abnormalities (%);
number of error/total events | Significant level | Standard deviation and
standard error |
| | HT 1080 cells | | Control pIneo2 | 9.7 (136/1400) | NA | 3.36/0.93 | | pI-17α | 21.3 (170/800) | <0.0001 | 7.11/2.51 | | pI-21α | 21.3 (128/600) | <0.0001 | 5.17/2.11 | | pI-Yα | 25.3 (76/300) | <0.0001 | 1.15/0.66 | | pI-DYZ1 | 7.5 (90/1200) | Not significant | 2.90/0.83 | | pI-Major | 10.6 (74/700) | Not significant | 3.34/1.26 | | pI-Minor | 13.6 (95/700) | Not significant | 3.47/1.37 | | HUES-10 cells | | Control pIneo2 | 5.0 (15/300) | NA | 4.58/2.65 | | pI-17α | 18.7 (56/300) | <0.02 | 6.51/3.76 | | pI-21α | 19.7 (59/300) | <0.029 | 8.5/4.91 | | pI-Yα | 16.3 (49/300) | <0.014 | 1.15/0.67 | | pI-DYZ1 | 6.7 (20/300) | Not significant | 3.79/2.19 | | pI-Major | 6.3 (19/300) | Not significant | 4.93/2.85 | | pI-Minor | 5.7 (17/300) | Not significant | 7.23/4.18 | | mES cells | | Control pIneo2 | 4.7 (14/300) | NA | 2.52/1.45 | | pI-17α | 6.0 (18/300) | Not significant | 2.00/1.15 | | pI-21α | 6.0 (18/300) | Not significant | 2.00/1.15 | | pI-Yα | 7.3 (22/300) | Not significant | 0.58/0.33 | | pI-DYZ1 | 6.0 (18/300) | Not significant | 3.61/2.08 | | pI-Major | 7.3 (22/300) | Not significant | 2.89/1.67 | | pI-Minor | 14.3 (43/300) | <0.025 | 5.51/3.18 |
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