Figure 3: Overexpression of FBXW7 can induce epithelial-mesenchymal transition (EMT). (a) The expression of EMT markers of E-cadherin, N-cadherin, Vimentin, and Snail1 was analyzed by Western blotting. GAPDH was probed as the loading control. (b) Immunofluorescence staining analyzed EMT marker Vimentin (green), and the nuclei were stained with DAPI (blue). (c) mRNA expression level of Twist1 was detected by qRT-PCR analysis. All results are from the three independent experiments. based on the Student t-test. Data are represented as mean ± SD.