miR-195 Regulates Proliferation and Apoptosis through Inhibiting the mTOR/p70s6k Signaling Pathway by Targeting HMGA2 in Esophageal Carcinoma Cells

Figure 2

miR-195 directly targets the 3′-UTR of HMGA2 to regulate HMGA2 expression in EC cell lines. (a) Prediction of the HMGA2 mRNA 3′-UTR sites targeted by miR-195. The underline showed the mutated nucleotides on the 3′-UTR of HMGA2. Relative luciferase activity in (b) EC109 and (c) EC9706 cells. Cells were transfected with pMIR-REPORT vector containing HMGA2-3′-UTR-wt or HMGA2-3′-UTR-mut together with miR-195 mimics or miR-control for 24 h. Renilla activity was used as the internal control. The expressions of HMGA2 mRNA in (d) EC109 and (e) EC9706 cells transfected with miR-195 mimics, anti-miR-195, or miR-control were detected by qRT-PCR. The expressions of HMGA2 protein in (f and g) EC109 and (h and i) EC9706 cells transfected with miR-195 mimics, anti-miR-195, or miR-control were detected by western blot. The expressions of HMGA2 mRNA and protein were normalized to U6 snRNA and β-actin. Values are presented as the mean ± SD, . , .

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