Disease Markers / 2017 / Article / Fig 3

Research Article

miR-195 Regulates Proliferation and Apoptosis through Inhibiting the mTOR/p70s6k Signaling Pathway by Targeting HMGA2 in Esophageal Carcinoma Cells

Figure 3

Overexpression of HMGA2 relieved the effect of miR-195 on proliferation and apoptosis in EC cell lines. EC109 and EC9706 cells were transfected with miR-control or miR-195 mimics or cotransfected miR-195 mimics with pcDNA or pcDNA-HMGA2. The protein expressions of HMGA2 in EC109 (a) and EC9706 (b) cells were detected by western blot. Cell proliferation in EC109 (c) and EC9706 (d) cells was assessed by MTT assay at 24 and 48 h. Cell apoptosis in EC109 (e) and EC9706 (f) cells was detected by flow cytometry via double staining of cells with Annexin V-FITC and propidium iodide (PI) at 48 h. Values are presented as the mean ± SD, . , .