miR-195 Regulates Proliferation and Apoptosis through Inhibiting the mTOR/p70s6k Signaling Pathway by Targeting HMGA2 in Esophageal Carcinoma Cells

Figure 4

miR-195 suppressed the mTOR/p70s6k signaling pathway by inhibiting HMGA2 expression in EC9706 cells. (a and b) Western blot analysis for relative proteins p-mTOR, t-mTOR, p-p70s6k, and t-p70s6k in EC9706 cells transfected with miR-control or miR-195 mimics or cotransfected miR-195 mimics together with pcDNA or pcDNA-HMGA2. (c and d) Western blot analysis for relative proteins p-mTOR, t-mTOR, p-p70s6k, and t-p70s6k in EC9706 cells transfected with anti-miR-control or anti-miR-195 or cotransfected anti-miR-195 together with si-control or si-HMGA2. β-actin was used as an internal control. Values are presented as the mean ± SD, . .

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