EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

Figure 1

EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μl of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β-actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

(a)

(b)