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Disease Markers
Volume 2018, Article ID 1371425, 10 pages
https://doi.org/10.1155/2018/1371425
Research Article

Association of Maternal and Fetal Single-Nucleotide Polymorphisms in Metalloproteinase (MMP1, MMP2, MMP3, and MMP9) Genes with Preeclampsia

1Department of Medical Biotechnology, Medical University of Lodz, Łódź, Poland
2Department of Obstetrics, Perinatology and Gynecology, Polish Mother’s Memorial Hospital Research Institute, Łódź, Poland
3Department of Obstetrics and Perinatology, University Hospital in Kraków, Kraków, Poland
4Department of Genetics, Polish Mother’s Memorial Hospital-Research Institute, Łódź, Poland
5Department of Microelectronics and Computer Science, Lodz University of Technology, Łódź, Poland

Correspondence should be addressed to Agata Sakowicz; moc.liamg@zciwokas.ataga

Received 23 August 2017; Revised 30 October 2017; Accepted 11 January 2018; Published 18 February 2018

Academic Editor: Yi-Chia Huang

Copyright © 2018 Agata Sakowicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Metalloproteinases (MMPs) play a pivotal role during the process of trophoblast invasion and placentation. The appearance of five functional single-nucleotide polymorphisms (SNP) in the genes of the metalloproteinases most commonly implicated in the implantation process may influence the development of preeclampsia. Methods. Blood samples were collected from 86 mothers and 86 children after preeclampsia and 85 mothers and 85 children with uncomplicated pregnancies. The distribution of genotypes for −1607 1G/2G MMP1, −735 C/T MMP2, −1306 C/T MMP2, −1171 5A/6A MMP3, and −1562C/T MMP9 polymorphisms was determined by RFLP-PCR. Results. The occurrence of 1G/1G MMP1 or 5A/5A MMP3 genotype in the mother or 1G/1G MMP1 or 5A/6A MMP3 genotype in the child is associated with preeclampsia development. Moreover, simultaneous maternal and fetal 1G/1G homozygosity increases the risk of preeclampsia development 2.39-fold and the set of maternal 5A/5A and fetal 5A/6A MMP3 genotypes by over 4.5 times. No association between the carriage of studied MMP2 or MMP9 polymorphisms and the predisposition to preeclampsia was found. Conclusion. The maternal 1G/1G MMP1 and 5A/5A MMP3 and fetal 1G/1G MMP1 and 5A/6A MMP3 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together.