Research Article
Positive Association between EDN1 rs5370 (Lys198Asn) Polymorphism and Large Artery Stroke in a Ukrainian Population
Table 1
PCR conditions for EDN1 rs5370 and EDNRA rs5335 genotyping.
| | Gene | SNP | Primer nucleotide sequence | Thermocycling conditions | PCR amplicon size | | D | H | E |
| | EDN1 | rs5370 | F: 5-TCTTGCTTTATTAGGTCGGAGACC-3
R: 5-TTTGAACGAGGACGCTGGTC-3 | 94°С (60 s) | 61°С (60 s) | 72°С (45 s) | 262 bp |
| | EDNRA | rs5335 | F: 5-TAGAAGCACTCCTCGGTACTCC-3
R: 5-TCG TAGATGTTGTGGGTGGATA-3 | 94°С (50 s) | 60°С (40 s) | 72°С (60 s) | 174 bp |
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Annotation: F: forward; R: reverse; D: denaturation; H: hybridization; E: elongation; bp: base pairs.
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