CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer

Figure 4

Functional assays of EGFR gene-edited SW579 cells: (a) The image shows the cell cycle distribution of SC sgRNA- and EGFR sgRNA_2-infected SW579 cells by flow cytometry analysis. (b) SW579 cell proliferation of SC sgRNA- and EGFR sgRNA_2-infected SW579 cells was observed for 4 days and determined by MTT assay. (c) Colony formation of SC sgRNA- and EGFR sgRNA_2-infected SW579 cells was measured by 0.5% crystal violet staining. (d) The cell colony numbers for both SC sgRNA- and EGFR sgRNA_2-infected SW579 cells were counted by ImageJ and presented in the percentage of SC control. (e) Q-PCR analysis on metastasis-related gene expressions, such as TWIST, SNAIL, VIMEMTIN, and MMP1, on SC sgRNA- and EGFR sgRNA_2-infected SW579 cells. All the gene expressions were normalized by their GUS gene level and presented in the percentage of SC. The statistical significance between groups is compared by Student’s t-test. The value less than 0.01 is presented with two asterisks.

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