CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer

Figure 5

Afatinib inhibits SW579 cell survival and induces apoptosis. (a) The image shows the time-dependent manners of IC50 in afatinib-treated MDA-MB-231 cells by MTT assay at 24, 48, and 72 hours. (b) The LIVE/DEAD cell viability assay was performed after 1 and 10 μM afatinib treatment of MDA-MB-231 cells for 48 hours. Cells were subjected to the viability assay to identify live (green) and dead (red) cells. (c) Cell death after 1 and 10 μM afatinib treatment was analyzed for significance. (d) Afatinib significantly induced tumor-suppressive protein of P53 and cleaved PARP protein expression in a dose-dependent manner, as evidenced by western blot analysis. (e) The image shows the cell cycle distribution of afatinib-treated SW579 cells by flow cytometry analysis. Data are presented as the mean and standard error. Data were analyzed with Student’s t-test; all values were two-sided. values less than 0.05 are indicated with an asterisk; less than 0.01 is presented with two asterisks.

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