|
| Study | Cell lines investigated | HuR expression | HuR modification and activity |
|
| OSCCs | | | |
| Hasegawa et al. [66] | HSC3, Ca9-22 | Presence of cytoplasmic | (i) HuR knockdown (via siRNAs) ↓ c-myc export and accumulation
(ii) LMB after 7 h did not achieve HuR knockdown
(iii) s-fos and c-myc stabilisation |
| Cha et al. [63] | YD9, Y10B, Y32, Y38, HSC2, HSC3, and Ca9-22 | Predominantly cytoplasmic | (i) HuR knockdown (via LMB and siRNAs) ↓ cytoplasmic HuR and COX-2 induction |
| Cha et al. [64] | YD9, Y10B, Y32, Y38, HSC2, HSC3, and Ca9-22 | Predominantly cytoplasmic | (i) HuR knockdown (via LMB and siRNAs) ↓ cytoplasmic HuR and cIAP2 (concentration dependent) |
| Kakuguchi et al. [21] | HSC3, Ca9-22 | High expression | HuR knockdown (via siRNAs)
(i) ↓ cytoplasmic HuR
(ii) ↓ cytoplasmic c-fos, c-myc, and COX-2
(iii) ↓ cyclin A, B1, D1, and CDK1 expression
(iv) ↓ average invasion rate of cells (Matrigel invasion assay)
(v) ↑ loss of ability for anchorage-independent cell growth |
| Hwang et al. [65] | YD10B | Presence of expression | (i) HuR knockdown (via shRNAs) ↓ cytoplasmic HuR and MMP-9
(ii) KPS-A controls HuR expression via ERK and PI3K/AKT activation under hypoxia |
| Talwar et al. [67] | UM74B | Overexpression of HuR-CP1 | (i) HuR-CP1 associates with c-myc mRNA thus ↓ its translation
(ii) HuR knockdown (via siRNAs) ↑ c-myc expression |
|
| Thyroid lesions |
| Baldan et al. [97] | Nthy-ori-3.1, BCPAP, K1 TPC1, FTC133 WRO, FRO, and SW1736 | Overexpression in all PTCs and in SW1736 | (i) HuR knockdown (via siRNAs)
(a) ↑ apoptotic cells
(b) ↑ distinct gene expression modifications in BCPAP and Nthy-ori-3.1 cell lines
(ii) Different HuR-bound RNA profiles among BCPAP, K1, TPC1 and Nthy-ori-3.1 |
| Human oesophageal epithelial cells |
| Donahue et al. [101] | Derived from human specimens | | Regulates survivin, in the absence of p53 |
|
