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Disease Markers
Volume 2018 (2018), Article ID 5812802, 12 pages
Research Article

A Novel Polymorphism in the Promoter of the CYP4A11 Gene Is Associated with Susceptibility to Coronary Artery Disease

1Department of Biology, Medical Genetics and Ecology, Kursk State Medical University, Karl Marx Street 3, Kursk 305041, Russia
2Department of Internal Medicine, Kursk State Medical University, 14 Pirogova St., Kursk 305035, Russia
3Laboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, Yamskaya Street 18, Kursk 305041, Russia
4Evolutionary Genetics Laboratory, Research Institute of Medical Genetics, Tomsk National Research Medical Center, 10 Nabereznaya Ushaiki, Tomsk 634050, Russia
5Department of Medical Biological Disciplines, Belgorod State University, 85 Pobeda St., Belgorod 308015, Russia
6Laboratory of Statistical Genetics and Bioinformatics, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 18 Yamskaya St., Kursk 305041, Russia

Correspondence should be addressed to Alexey Polonikov

Received 11 September 2017; Revised 23 October 2017; Accepted 29 November 2017; Published 1 February 2018

Academic Editor: Agata M. Bielecka-Dabrowa

Copyright © 2018 Svetlana Sirotina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Enzymes CYP4A11 and CYP4F2 are involved in biosynthesis of vasoactive 20-hydroxyeicosatetraenoic acid and may contribute to pathogenesis of coronary artery disease (CAD). We investigated whether polymorphisms of the CYP4A11 and CYP4F2 genes are associated with the risk of CAD in Russian population. DNA samples from 1323 unrelated subjects (637 angiographically confirmed CAD patients and 686 age- and sex-matched healthy individuals) were genotyped for polymorphisms rs3890011, rs9332978, and rs9333029 of CYP4A11 and rs3093098 and rs1558139 of CYP4F2 by using the Mass-ARRAY 4 system. SNPs rs3890011 and rs9332978 of CYP4A11 were associated with increased risk of CAD in women: OR = 1.26, 95% CI: 1.02–1.57, , and and OR = 1.45, 95% CI: 1.13–1.87, , and , respectively. Haplotype G-C-A of CYP4A11 was associated with increased risk of CAD (adjusted OR = 1.41, 95% CI: 1.12–1.78, and ). Epistatic interactions were found between rs9332978 of CYP4A11 and rs1558139 of CYP4F2 (). In silico analysis allowed identifying that SNP rs9332978 is located at a binding site for multiple transcription factors; many of them are known to regulate the pathways involved in the pathogenesis of CAD. This is the first study in Europeans that reported association between polymorphism rs9332978 of CYP4A11 and susceptibility to coronary artery disease.