Correlation between miR-200 Family Overexpression and Cancer Prognosis

Table 3

Stratified analysis of the high expression of the miR-200 family and overall survival.

Categories	Subgroups	Univariate analyses					Multivariate analyses
		Number of datasets	(95% CI)	value	^2	Ph	Number of datasets	(95% CI)	value	^2	Ph
All		19	1.32 (1.14–1.54)	<0.001	77.50%	<0.001	24	1.32 (1.16–1.49)	<0.001	75.10%	<0.001
Patient source	Asia	10	1.91 (1.26–2.92)	0.003	80.10%	<0.001	13	1.98 (1.34–2.90)	0.001	78.20%	<0.001
	Europe	5	1.07 (0.95–1.21)	0.286	66.80%	<0.001	8	1.11 (0.99–1.24)	0.071	67.10%	<0.001
	North America	4	3.81 (2.46–5.90)	<0.001	0.00%	0.685	3	2.37 (1.44–3.91)	0.001	0.00%	0.457
Cancer type	EOC	7	2.18 (1.23–3.86)	0.008	79.90%	<0.001	7	1.98 (1.03–3.80)	0.039	81.80%	<0.001
	CRC	7	1.12 (0.96–1.31)	0.140	77.70%	<0.001	8	1.15 (1.02–1.30)	0.026	60.10%	0.001
	NSCLC	3	3.36 (1.64–6.89)	0.001	0.00%	0.411	6	2.91 (1.99–4.26)	<0.001	33.40%	0.185
	GC	1	0.94 (0.75–1.17)	0.565	1.90%	0.361	2	1.10 (0.72–1.68)	0.669	62.30%	0.047
	BC	1	1.38 (1.11–1.71)	0.003	/	/	1	1.46 (0.54–3.91)	0.454	75.10%	0.045
Test method	RT-PCR	16	1.64 (1.24–2.16)	0.001	75.30%	<0.001	19	1.57 (1.23–1.99)	<0.001	75.10%	<0.001
	ISH	1	21.42 (7.54–60.83)	<0.001	0.00%	0.996	1	16.28 (6.28–42.24)	<0.001	0.00%	0.995
	TaqMan	2	1.01 (0.95–1.08)	0.686	47.70%	0.046	4	1.07 (0.95–1.20)	0.249	58.80%	0.005
Sample source	FFPE	2	0.57 (0.29–1.10)	0.095	0.00%	0.890	5	2.27 (1.56–3.32)	<0.001	43.10%	0.135
	Tissue	5	3.19 (1.19–8.52)	0.021	84.40%	<0.001	9	2.04 (1.13–3.68)	0.017	80.70%	<0.001
	Blood	10	1.34 (1.15–1.57)	<0.001	79.00%	<0.001	9	1.14 (1.02–1.28)	0.019	68.30%	<0.001
	TMA	2	0.93 (0.75–1.16)	0.527	0.00%	0.519	1	0.94 (0.73–1.21)	0.649	40.90%	0.184
Sample size	≧100	11	1.25 (1.06–1.47)	0.007	78.90%	<0.001	14	1.29 (1.11–1.49)	0.001	71.60%	<0.001
	<100	8	1.74 (1.10–2.75)	0.018	68.50%	0.001	10	1.84 (1.17–2.90)	0.008	79.60%	<0.001
miR-200 component	miR-200a	7	1.14 (0.81–1.61)	0.438	64.80%	0.009	6	1.07 (0.72–1.59)	0.723	78.30%	<0.001
	miR-200b	6	1.38 (0.88–2.16)	0.166	82.10%	<0.001	6	1.36 (0.89–2.08)	0.158	76.70%	0.001
	miR-200c	11	1.38 (1.01–1.89)	0.040	82.40%	<0.001	10	1.62 (1.12–2.33)	0.010	79.30%	<0.001
	miR-141	7	2.01 (1.26–3.21)	0.003	83.50%	<0.001	7	1.24 (0.99–1.56)	0.060	68.00%	0.005
	miR-429	5	0.99 (0.73–1.34)	0.953	62.20%	0.032	8	1.41 (1.01–1.98)	0.043	70.30%	0.001

EOC: epithelial ovarian cancer; BC: breast cancer; NSCLC: nonsmall cell lung cancer; GC: gastric cancer; CRC: colorectal cancer; RT-PCR: reverse transcription-polymerase chain reaction; ISH: in situ hybridization; FFPE: formalin-fixed and paraffin-embedded; TMA: tissue microarray; : hazard ratio; CI: confidence interval; Ph: value of the heterogeneity test.