Long Noncoding RNA TUG1/miR-29c Axis Affects Cell Proliferation, Invasion, and Migration in Human Pancreatic Cancer

Figure 3

miR-29c was downregulation in PC tissues and bound to TUG1. (a) The expression of miR-29c in human pancreatic cancer tissues was significantly lower than that in peritumoural normal tissues by the qRT-PCR method. (b) Correlation between TUG1 and miR-29c in pancreatic cancer tissues. (c) qRT-PCR was performed to determine the expression of miR-29c in BxPC-3 and PANC-1 cells after Lv-TUG1 shRNA transfection. (d) The predicted binding sequences of miR-29c in TUG1. Mutation was generated in the seed region (red bases) of TUG1. (e, f) The relative luciferase activity was inhibited in BxPC-3 (e) and PANC-1 (f) cells cotransfected with wild-type (wt) TUG1 3UTR and miR-29c; however, the relative luciferase activity was not inhibited in cells transfected with mutant-type (mut) TUG1 3UTR. Firefly luciferase activity was normalised to Renilla luciferase. vs. NC group.

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