Disease Markers

Research Article

Differential Gene Expression Profile of Renin-Angiotensin System in the Left Atrium in Mitral Regurgitation Patients

Table 3

Comparison of mRNA levels through quantitative PCR in the left atria among MR patients with heart failure, aortic valve disease patients with heart failure, and normal controls.


Gene name	MR ()	AVD ()	NC ()	value
Gene name	MR ()	AVD ()	NC ()	MR vs. NC	AVD vs. NC	MR vs. AVD

ACE	12.22 ± 0.48	12.37 ± 0.31	9.85 ± 0.66	0.023	0.005	0.657
ACE2	11.61 ± 0.69	12.83 ± 0.34	7.57 ± 0.32	0.003	0.002	0.149
AT1	13.27 ± 0.36	14.05 ± 0.47	12.04 ± 0.29	0.013	0.010	0.126
ENPEP	8.86 ± 0.24	9.52 ± 0.31	8.14 ± 0.10	0.007	0.002	0.068
AGT	5.18 ± 0.28	4.91 ± 0.25	4.09 ± 0.22	0.007	0.053	0.594
CTSA	6.24 ± 0.25	5.13 ± 0.19	4.44 ± 0.31	0.003	0.071	0.006
THOP1	9.47 ± 0.42	8.83 ± 0.24	7.89 ± 0.08	0.002	0.007	0.424
NLN	10.60 ± 0.29	10.00 ± 0.18	8.72 ± 0.30	0.005	0.020	0.214
ANPEP	10.52 ± 0.55	10.66 ± 0.73	7.03 ± 0.52	0.001	0.005	0.923
CTSG	11.71 ± 0.48	11.73 ± 0.47	9.94 ± 0.59	0.042	0.042	0.949
LNPEP	7.91 ± 0.55	6.17 ± 0.27	5.18 ± 0.40	0.005	0.039	0.013
MME	11.08 ± 0.54	10.66 ± 0.23	7.88 ± 0.66	0.010	0.002	0.441
CMA1	13.88 ± 0.46	13.43 ± 0.38	11.50 ± 0.94	0.063	0.053	0.298
CPA3	11.30 ± 0.77	10.59 ± 0.41	7.64 ± 0.71	0.007	0.014	0.441
MAS1	6.35 ± 0.90	12.19 ± 1.44	16.39 ± 0.69	0.001	0.028	0.016
NR3C2	4.89 ± 0.14		6.08 ± 0.31	0.007
CYP11B2	4.56 ± 0.28		Unmeasurable

Data are presented as mean ± SEM; quantitative RT-PCR values are presented in △Cq units; MR: mitral regurgitation; AVD: aortic valve disease; NC: purchased samples from normal subjects. ACE: angiotensin-converting enzyme; ACE2: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; AT1: angiotensin II receptor, type 1; ENPEP: glutamyl aminopeptidase; AGT: angiotensinogen; CTSA: cathepsin A; THOP1: thimet oligopeptidase 1; NLN: neurolysin, mitochondrial-like; ANPEP: alanyl (membrane) aminopeptidase; CTSG: cathepsin G; LNPEP: leucyl/cystinyl aminopeptidase; MME: neprilysin; CMA1: mast cell protease 2-like; CPA3: carboxypeptidase A3; MAS1: MAS1 oncogene; NR3C2: mineralocorticoid receptor; CYP11B2: aldosterone synthase gene.