Knockdown of Heparanase Suppresses Invasion of Human Trophoblasts by Activating p38 MAPK Signaling Pathway

Figure 3

Effect of HPSE on trophoblast cell proliferation and apoptosis. (a) The rate of cell viability in 24 h, 48 h, and 72 h after seeding. HTR8 as a reference. Cells were conventionally cultured for 48 h, harvested with 0.25% trypsin without EDTA, and double-stained with annexin V-APC/7-AAD for flow analysis. (b–f) Flow cytometry analysis of cell apoptosis. (b) HTR8, (c) pLenti-HTR8, (d) pLenti-HPSE-HTR8, (e) shRNA-HTR8, and (f) shRNA-HPSE-HTR8. 1: viable cells. 2: early apoptotic cells. 3: late apoptotic cells. 4: necrotic cells. (g) The percentage of apoptosis cells in five cell lines. Data in graph a and graph g were represented as the mean ± SD. The differences among HTR8, pLenti-HTR8, and shRNA-HTR8, between pLenti-HTR8 and pLenti-HPSE-HTR8, and between shRNA-HTR8 and shRNA-HPSE-HTR8 were compared by one-way ANOVA and Holm-Sidak’s post hoc test. ; .

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