Microtubule-Associated Protein 4 Is a Prognostic Factor and Promotes Tumor Progression in Lung Adenocarcinoma
MAP4 promotes LADC cell invasion in vitro. The matrigel transwell assay was performed in A549 and H1299 cells transfected with MAP4 siRNA or a negative control to determine cell invasion potential. The number of cells migrated to the bottom were calculated manually. MAP4 knockdown repressed the cell invasion in both two types of cells. (a) Representative photos of invasive cells. (b) Quantification analysis of the invasive cell number per field. Data represent mean + SD of duplicates from three fields of view (, Student’s t-test).