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Disease Markers
Volume 2018, Article ID 9369358, 9 pages
https://doi.org/10.1155/2018/9369358
Research Article

Expression of Pentose Phosphate Pathway-Related Proteins in Breast Cancer

1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Republic of Korea
2Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea

Correspondence should be addressed to Ja Seung Koo; ca.shuy@6791sjk

Received 20 June 2017; Accepted 17 January 2018; Published 25 February 2018

Academic Editor: Fabrizia Bamonti

Copyright © 2018 Junjeong Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. The purpose of this study was to assess the expression of pentose phosphate pathway- (PPP-) related proteins and their significance in clinicopathologic factors of breast cancer. Methods. Immunohistochemical staining for PPP-related proteins (glucose-6-phosphate dehydrogenase [G6PDH], 6-phosphogluconolactonase [6PGL], 6-phosphogluconate dehydrogenase [6PGDH], and nuclear factor-erythroid 2-related factor 2 [NRF2]) was performed using tissue microarray (TMA) of 348 breast cancers. mRNA levels of these markers in publicly available data from the Cancer Genome Atlas project and Kaplan-Meier plotters were analyzed. Results. Expression of G6PDH and 6PGL was higher in HER-2 type ( and , resp.) and lower in luminal A type. 6PGDH expression was detected only in TNBC subtype (). G6PDH positivity was associated with ER negativity (), PR negativity (), and HER-2 positivity (), whereas 6PGL positivity was associated with higher T stage (). The 562 expression profile from the TCGA database revealed increased expression of G6PDH and 6PG in the tumor compared with normal adjacent breast tissue. The expression of G6PDH was highest in HER-2 type. HER-2 and basal-like subtypes showed higher expression of 6PGDH than luminal types. Conclusion. PPP-related proteins are differentially expressed in breast cancer according to molecular subtype, and higher expression of G6PDH and 6PGL was noted in HER-2 subtype.