NFE2L3 Inhibition Induces Cell Cycle Arrest at the G0/G1 Phase in Colorectal Cancer Cells through Downregulating CCND1 and pRb1-ser807/811

Figure 3

CCND1 mRNA and protein expression levels in colorectal cancer cell lines after NFE2L3 knockdown. (a) The mRNA expression levels of NFE2L3 in HCT116 cells after NFE2L3 knockdown. Inhibition of the NFE2L3 mRNA level was displayed in the si-1 and si-2 groups (, ). (b) CCND1 mRNA expression levels in HCT116 cells after NFE2L3 knockdown. Inhibition of CCND1 mRNA expression was detected in the si-1 and si-2 groups (, ). (c) Protein expression changes of CCND1 in HCT116 cells when inhibited by NFE2L3 siRNA. Downregulation of CCND1 and pRb1-ser807/811 proteins was observed in the si-1 and si-2 groups. (d) The mRNA expression levels of NFE2L3 in SW480 cells after NFE2L3 knockdown. The NFE2L3 mRNA expression level was significantly inhibited by si-1 and si-2 (, ). (e) CCND1 mRNA expression levels in SW480 cells after NFE2L3 knockdown. The CCND1 mRNA level was significantly downregulated in the si-1 group (). (f) Protein expression changes of CCND1 in SW480 cells after transfection with NFE2L3 siRNA. CCND1 and pRb1-ser807/811 protein expression levels were downregulated in the si-1 and si-2 groups.

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