Disease Markers

Research Article

Investigating the Protein Signature of Adamantinomatous Craniopharyngioma Pediatric Brain Tumor Tissue: Towards the Comprehension of Its Aggressive Behavior

Table 1

List and distribution of the proteins and peptides identified in AC tissue in at least four out of the seven samples analysed by LC-MS in top-down approach.
Uniprot accessionDescriptionMolecular weight [M+H]+Posttranslational modification (PTM)Sample distribution#
AC1AC2AC3AC4AC5AC6AC7
P62328Thymosin β4§ [18, 23]4961.51N-term acetylation•••••••
Thymosin β4 oxidized form§ [23]4977.51N-term acetylation; M6 oxidation•••••——
Thymosin β4 fragment 2-12§ [23]1304.61N-term acetylation••••••—
Thymosin β4 fragment 20-442829.42 •••••••
Thymosin β4 fragment 20-402485.28 •••——•—
Thymosin β4 fragment 27-441984.99 ••—••••
Thymosin β4 fragment 16-443285.73 •••••••
Thymosin β4 fragment 21-442701.33 •——••••
Thymosin β4 fragment 20-422613.34 •••—••—
Thymosin β4 fragment 20-392357.23 •••——•—
Thymosin β4 fragment 26-442113.08 ••—••—•
P63313Thymosin β10§ [23]4934.54N-term acetylation•—•••—•
Thymosin β10 fragment 2-42, des-IS§ [23]4734.43N-term acetylation, C-terminal truncation•—•••——
Thymosin β10 fragment 2-27§ [23]2964.51N-term acetylation•—•••——
P06454-2Prothymosin α isoform 2§ [23]11978.90N-term acetylation•••••••
P06454Prothymosin α fragment 2-29,Thymosin α1§ [23]3107.52N-term acetylation•—•••——
Prothymosin α fragment 2-36,Thymosin α11§ [23]3788.83N-term acetylation••••••—
Prothymosin α fragment 90-1112553.08 ••—•••—
P59665Alpha-defensin1§ [16, 17]3440.53Disulfide bonds C2-C30; C4-C19; C9-C29•••••••
P59665Alpha-defensin2§ [16, 17]3369.50Disulfide bonds C1-C29; C3-C18; C8-C28•••••••
P59666Alpha-defensin3§ [16, 17]3484.52Disulfide bonds C2-C30; C4-C19; C9-C29••—••••
P12838Alpha-defensin4§ [16, 17]3707.77Disulfide bonds C2-C30; C4-C19; C9-C29—•••—•—
P02671Fibrinogen alpha chain fragment 20-35, fibrinopeptide A1536.69 •••••••
P14136Glial fibrillary acidic protein fragment 388-4325206.73 ••—•——•
Glial fibrillary acidic protein fragment 398-4324035.12 ••—••—•
Glial fibrillary acidic protein fragment 406-4323197.67 ••—•——•
Glial fibrillary acidic protein fragment 416-4301797.91 ••—••—•
Glial fibrillary acidic protein fragment 414-4322288.11 ••—••—•
Glial fibrillary acidic protein fragment 406-4292852.55 •••••••
Glial fibrillary acidic protein fragment 389-4051900.01 ••—••——
Glial fibrillary acidic protein fragment 388-4052028.08 ••—••—•
Glial fibrillary acidic protein fragment418-4321756.89 ••—••—•
Glial fibrillary acidic protein fragment 414-4302058.00 ••—••—•
Glial fibrillary acidic protein fragment 416-4322028.02 ••—••••
Glial fibrillary acidic protein fragment 412-4322488.23 ••—••—•
Glial fibrillary acidic protein fragment 384-3971651.86 ••—•——•
Glial fibrillary acidic protein fragment 389-3981149.61 ••—••—•
Glial fibrillary acidic protein fragment 412-4312357.23 ••—••——
Glial fibrillary acidic protein fragment 401-4323761.98 ••—••——
Glial fibrillary acidic protein fragment 407-4323041.58 ••—•——•
Glial fibrillary acidic protein fragment 413-4322387.17 ••—•——•
Glial fibrillary acidic protein fragment 412-4302258.11 ••—••——
Glial fibrillary acidic protein fragment 415-4322159.06 •——••—•
Glial fibrillary acidic protein fragment 415-4301928.95 ••—••——
Glial fibrillary acidic protein fragment 417-4321871.92 ••—••—•
Glial fibrillary acidic protein fragment 419-4321699.87 ••—••——
Glial fibrillary acidic protein fragment 416-4291682.88 ••—•——•
Glial fibrillary acidic protein fragment 416-4281554.79 ••—••—•
Glial fibrillary acidic protein fragment 418-4301526.78 ••—••—•
Glial fibrillary acidic protein fragment 385-3971504.80 ••—••——
Glial fibrillary acidic protein fragment 389-4011464.76 ••—••——
Glial fibrillary acidic protein fragment 386-3971417.77 ••—•——•
P08670Vimentin fragment 434-4663814.94 —•••—•—
Vimentin fragment 445-4662551.21 ••••—•—
Vimentin fragment 444-4662664.29 •••••••
Vimentin fragment 443-4662777.38 •••••••
Vimentin fragment 446-4662423.11 ••••••—
Vimentin fragment 450-4661992.90 •••••••
Vimentin fragment 13-28 or 12-271650.82 —•••—•—
Vimentin fragment 440-4663147.60 ••—•—•—
Vimentin fragment 447-4662322.06 ••••—•—
Vimentin fragment 441-4662991.51 ••••••—
Vimentin fragment 442-4662890.46 ••—•—•—
Vimentin fragment 5-282504.21 ••••———
Vimentin fragment 29-502391.23 ••••———
Vimentin fragment 424-4422195.19 ••—•—•—
Vimentin fragment 451-4661836.80 ••—•—•—
Vimentin fragment 452-4661721.77 —•—•••—
Vimentin fragment 453-4661664.76 ••—••——
Vimentin fragment 454-4661536.69 ••—•••—
P0CG47Ubiquitin§ [14]8560.64 •••••••
Ubiquitin fragment 1-74, des-GG§ [23]8446.60C-terminal truncation••••••—
Ubiquitin fragment 1-73, des-RGG§ [23]8290.51C-terminal truncation—••—••—
P06703Protein S100-A6 cysteinylated form§ [17, 52]10204.33N-term acetylation; cysteinylation••••—••
P31949Protein S100-A11§ [52, 53]11644.79N-term acetylation•—••—•—
P69905Hemoglobin subunit alpha fragment 2-343473.77 •••••••
Hemoglobin subunit alpha fragment 2-333326.70 —•••—•—
Hemoglobin subunit alpha fragment 111-1423427.83 ••••••—
Hemoglobin subunit alpha fragment 107-1423854.12 ••—•—•—
Hemoglobin subunit alpha fragment 110-1423540.91 —•••—•—
Hemoglobin subunit alpha fragment 16-341992.97 ••—•—•—
Hemoglobin subunit alpha fragment 34-471732.87 ——••••—
P68871Hemoglobin subunit beta fragment 131-1471869.01 ——••••—
Hemoglobin subunit beta fragment 33-42, LVV-hemorphin-71308.71 ••••—•—
P0DP23Calmodulin§ [52]16780.912 acetylations (or 1 acetylation and 1 trimethylation)•••••••
P01011Alpha-1-antichymotrypsin fragment 387-4234352.34C-terminal fragment•••••••
Alpha-1-antichymotrypsin fragment 390-4234023.19C-terminal fragment••••——•
Alpha-1-antichymotrypsin fragment 384-4234623.52C-terminal fragment—•—•—••
P01009Alpha-1-antitrypsin fragment 383-4184133.24C-terminal fragment—•—•—••
Alpha-1-antitrypsin fragment 384-4184046.20C-terminal fragment••••—••
P80723Brain acid-soluble protein 1 fragment 199-2272892.43C-terminal fragment——•—•••
P55087Aquaporin-4 fragment 275-3033257.61 ••—••—•
P02768Serum albumin fragment 25-482753.44 —•••—••
P16403; P16402; P10412Histone H1.2 fragment 33-53/histone H1.3 fragment 34-54/histone H1.4 fragment 33-532139.21 ••••—•—
P05204Non-histone chromosomal protein HMG-17 fragment 29-421471.86 •—••—•—
O60814Histone H2B type 1-K fragment 2-121092.61 ••—•••—
Histone H2B type 1-K fragment 2-161492.85 ••—••——
Q5QNW6Histone H2B type 2-F fragment 2-171606.93 ••—••——
P17677Neuromodulin fragment 220-2382154.92 ••—••——
P46821Microtubule-associated protein 1B fragment 853-8742624.36 ••—••——
P68363Tubulin alpha-1B fragment 68-791384.73 ••—••——
§Based on previous identification, relative reference reported. In bold are marked the protein/peptides identified in at least six of the seven samples analyzed. Monoisotopic. #The “•” and “-” symbols indicate detected and undetected proteins/peptides, respectively.