Biological Analysis of Gene Expression and Clinical Variables Suggest FZD1 as a Novel Biomarker for Patients with Kashin-Beck Disease, an Endemic Osteoarthritis in China

Table 4

List of 39 differentially expressed genes in KBD patients.

Gene name	Symbol	Public ID	Fold change^a
Downregulated genes
ATP-binding cassette, subfamily C, member 13, pseudogene	ABCC13	NR_003087	0.42 ± 0.03
ABI family, member 3 (NESH) binding protein	ABI3BP	NM_015429	0.33 ± 0.03
Acyl-CoA synthetase long-chain family member 6	ACSL6	NM_001009185	0.31 ± 0.01
Anaphase promoting complex subunit 1	ANAPC1	XM_006712690	0.33 ± 0.02
Branched chain amino-acid transaminase 1, cytosolic	BCAT1	NM_001178091	0.43 ± 0.04
Chromosome 1 open reading frame 64	C1orf64	NM_178840	0.43 ± 0.03
Calcium channel, voltage-dependent, gamma subunit 6	CACNG6	NM_145814	0.39 ± 0.02
Chondroitin sulfate N-acetylgalactosaminyltransferase 1	CSGALNACT1	NM_001130518	0.45 ± 0.03
Cathepsin C	CTSC	NM_001114173	0.39 ± 0.02
Cytochrome b5 reductase 3	CYB5R3	NM_000398	0.48 ± 0.04
Dystrophin, muscular dystrophy	DMD	NM_007868	0.37 ± 0.02
Enhancer of rudimentary homolog (Drosophila)	ERH	NM_004450	0.38 ± 0.02
F11 receptor	F11R	NM_016946	0.46 ± 0.02
Fibrillin 1	FKBP9	NM_007270	0.49 ± 0.06
Frizzled family receptor 1	FZD1	NM_003505	0.47 ± 0.03
Growth differentiation factor 5	GDF5	NM_000557	0.44 ± 0.03
Glutaredoxin 5	GLRX5	NM_016417	0.46 ± 0.04
Hemoglobin, alpha 2	HBA2	NM_000517	0.49 ± 0.03
Mitochondrial carrier triple repeat 3 pseudogene	MCART3P	NR_026540	0.43 ± 0.03
STE20-related kinase adaptor beta	STRADB	NM_001206864	0.43 ± 0.03
Zic family member 5	ZIC5	NM_033132	0.36 ± 0.02
Upregulated genes
Aquaporin 1	ATR	NM_001184	2.84 ± 0.27
Baculoviral IAP repeat-containing 3	BIRC3	NM_001165	4.26 ± 0.35
Collagen, type I, alpha 1	COL1A1	NM_000088	2.44 ± 0.44
CD3g molecule, gamma (CD3-TCR complex)	CD3G	NM_000073	3.06 ± 0.25
EYA transcriptional coactivator and phosphatase 4	EYA4	NM_001301012	3.77 ± 0.53
Fem-1 homolog a (C. elegans)	FEM1A	NM_018708	2.68 ± 0.49
F-box protein 15	FBXO15	NM_001142958	2.59 ± 0.18
FGFR1 oncogene partner 2	FGFR1OP2	NM_001171887	3.19 ± 0.24
Small subunit (SSU) processed component, homolog	KRR1	NM_007043	2.25 ± 0.19
Optineurin	OPTN	NM_001008211	2.01 ± 0.14
Phosphodiesterase 8B	PDE8B	XM_005248623	2.79 ± 0.16
Required for meiotic nuclear division 5 homolog A	RMND5A	NM_0022780	2.90 ± 0.43
Sialic acid binding Ig-like lectin 8	SIGLEC8	NM_014442	2.49 ± 0.29
Solute carrier family 14 (urea transporter), member 1	SLC14A1	NM_001128588	2.47 ± 0.22
Spermatogenesis associated 22	SPATA22	NM_001170695	2.06 ± 0.21
Single-stranded DNA binding protein 1, mitochondrial	SSBP1	NM_001256510	3.12 ± 0.25
Tumor necrosis factor (ligand) superfamily, member 11	TNFSF11	NM_003701	3.30 ± 0.53
Tetratricopeptide repeat domain 25	TTC25	NM_031421	2.52 ± 0.15

Differential expression genes between the KBD patients vs. controls were assessed using the selection criteria described in the Materials and Methods. According to the fold change value, only those genes showing significant differences ( value < 0.05) in expression, screening for the differential expression genes in KBD, are listed. ^aFold change, the mean and standard error of the mean (SEM) of the fold change in the expression of each gene.