Glucose-Regulated Protein 94 Modulates the Response of Osteosarcoma to Chemotherapy

Figure 3

GRP94-KD inhibited chemotherapy-induced apoptosis in OS cells. (a) Representative DAPI staining of MG63 cells. Scrambled control and GRP94-KD MG63 cells were incubated with paclitaxel (6 ng/mL), gemcitabine (4 μg/mL), or epirubicin (2 μg/mL) for 48 hr and then stained with DAPI. Apoptotic cells were imaged using a fluorescence microscope. (b) The populations of scrambled control and GRP94-KD 143B cells in different phases of the cell cycle and sub-G₁ populations after chemotherapy treatment. The flow cytometry analysis of the cell cycle was performed by staining DNA with PI. The results reported were obtained from at least three independently repeated experiments.

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