Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer

Figure 2

KIF20A can regulate the proliferation, migration, and invasion of prostate cancer cells. (a) Expression of KIF20A in different PCa cell lines. (b) KIF20A knockdown was performed in the C4-2 cell line; the efficiency of the plasmid was verified by Western blotting. (c) MTT assay was performed to evaluate proliferation in the C4-2 cell line transfected with shKIF20A plasmid. (d) Colony formation assay in the C4-2 cell line transfected with shKIF20A plasmid. (e) Transwell assay was performed to evaluate migration and invasion of the C4-2 cell line transfected with shKIF20A plasmid. (f) Effect of knocking down KIF20A on the expression of proliferation markers (Ki67, PCNA) by Western blotting. (g) Effect of knocking down KIF20A on the expression of metastasis markers (MMP2, MMP9) by Western blotting. Data represent . , , and .

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