KIF20A Affects the Prognosis of Bladder Cancer by Promoting the Proliferation and Metastasis of Bladder Cancer Cells

Figure 3

Knockdown of KIF20A inhibits the proliferation and invasion of bladder cancer cells. (a) Western blot was used to detect the protein expression levels of KIF20A in T24, BIU87, EJ, 5637, and SV-HUC-1 cells. (b, c) The shKIF20A plasmid was transfected into T24 and 5637 cells. The protein expression levels of KIF20A after transfection were detected by western blot. (d) Western blot was used to detect the expression levels of PCNA, Ki67, Bcl-2, caspase-3, and MMP-2 after the knockdown of KIF20A in T24 cells. (e) The MTT assay detected the growth of bladder cancer cells after the knockdown of KIF20A. The absorbance value was detected at a wavelength of 490 nm (). (f) A cloning formation assay detected the growth of bladder cancer cells after the knockdown of KIF20A. (g) The number of colonies in (g) was counted and plotted on a graph (). (h) Transwell invasion assays detected the invasiveness of the bladder cancer cells after the knockdown of KIF20A. (i) The number of invaded cells in (h) was counted and plotted on a graph ().

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