Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy

Figure 2

Immunoblot analysis of the livers demonstrated disrupted lipid metabolism in T. cruzi-infected mice and HFD further enhanced interruption of hepatic lipid metabolism during chronic Chagas disease. (a) T. cruzi-infected HFD-fed mice showed significantly reduced hepatic lipid metabolism compared to infected RD-fed mice as demonstrated by immunoblot analysis probed for various lipid metabolism markers such as fatty acid synthase (FAS), phosphorylated ATP-citrate lyase (pACL), phosphorylated acetyl co A carboxylase (p-ACC), carnitine palmitoyltransferase I (CPT1), and Lipin 1. Infected mice showed significantly increased hepatic levels of PPARα, a regulator of fatty acid oxidation compared to uninfected mice, and HFD further enhanced the levels of PPARα in the livers of infected mice. (b–h) Fold changes in the protein levels of FAS, pACL, acetyl CoA-synthase (AceCS1), pACC, CPT1, Lipin 1, and PPARα were normalized to GDI expression and represented as the bar graphs (b–h, respectively). The error bars represent the standard error of the mean. , , or compared to uninfected RD mice. ^#, ^##, or ^### compared to infected RD mice. ^{^}, ^{^^}, or ^{^^^} compared to uninfected HFD mice.

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