Circulating MicroRNAs as Novel Potential Biomarkers for Left Ventricular Remodeling in Postinfarction Heart Failure

Figure 5

Possible origins of plasma miR-20a-5p, miR-340-5p, and let-7i-5p. (a) The temporal expression of miR-20a-5p, miR-340-5p, and let-7i-5p in the PBMCs of post-MI rats and sham controls at 4, 8, and 12 weeks post-MI. (b) Transmission electron microscope image of exosomes isolated from plasma (A; white arrow) and western blot of exosomal markers ALIX, TSG101, and CD63 (B). (c) Expression levels of exosome-original miR-20a-5p, miR-340-5p, and let-7i-5p at 8 weeks post-MI. versus the corresponding sham group; ^Φ versus the corresponding sham group (miR-20a-5p); ^# versus the corresponding sham group (miR-340-5p); ^& versus the corresponding sham group (let-7i-5p). MI: myocardial infarction; PBMCs: peripheral blood mononuclear cells; ALIX: apoptosis-linked gene 2-interacting protein X; TSG101: tumour susceptibility gene 101 protein; CD63: cluster of differentiation 63.

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