DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma

Figure 2

DIAPH1 knockdown efficiency by shRNA lentivirus (SH). (a, d) Bright and fluorescent photomicrographs were taken 72 hours after lentivirus infection of DIAPH1 knockdown (SH) and the negative control (NC) in AMC-HN-8 cells (scale bars 50 μm) and FD-LSC-1 cells (scale bars 100 μm). (b, e) Identification of DIAPH1 knockdown efficiency in DIAPH1 SH and NC cells of the abovementioned cells by RT-qPCR. (c, f) Western blot analysis of DIAPH1 protein expression in the abovementioned cells.

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