Disease Markers / 2019 / Article / Fig 4

Research Article

Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors

Figure 4

Quality of small RNA isolated from exosomes. Representative electropherogram of RNA quality extracted from exosomes derived of (a) healthy donors and (b) BC patients. All samples were analyzed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) with the RNA 6000 Pico Kit (Agilent Technologies). The -axis represents fluorescence units (FU) and the -axis shows nucleotides (nt). The peak observed between 25 nt and 200 nt correspond to the small RNA region. Peaks in the regions around 1500 nt and 4000 nt relative to 18S and 28S ribosomal RNAs, respectively, were not or barely detected. The peak of 25 nt refers to the marker. (c) Virtual gel of the data is shown in the electropherograms.

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