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Disease Markers
Volume 2019, Article ID 7378967, 9 pages
Research Article

MicroRNA-224 Promotes Tumorigenesis through Downregulation of Caspase-9 in Triple-Negative Breast Cancer

1Department of Anesthesia, The Second Hospital of Jilin University, Changchun, Jilin, China
2Department of Hepatopancreatobiliary Surgery, The Second Hospital of Jilin University, Changchun, Jilin, China

Correspondence should be addressed to Miao He; moc.kooltuo@6101yhoaiM and Shixing Qiao; moc.kooltuo@88102oaiqgnixihs

Received 22 June 2018; Revised 5 November 2018; Accepted 26 November 2018; Published 11 February 2019

Academic Editor: Mirte Mayke Streppel

Copyright © 2019 Li Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Triple-negative breast cancer (TNBC) harbors genetic heterogeneity and generally has more aggressive clinical outcomes. As such, there is urgency in identifying new prognostic targets and developing novel therapeutic strategies. In this study, miR-224 was overexpressed in breast cancer cell lines and TNBC primary cancer samples. Knockdown of miR-224 in MDA-MB-231 cancer cells reduced cell proliferation, migration, and invasion. Through integrating in silico prediction algorithms with KEGG pathway and Gene Ontology analyses, CASP9 was identified to be a potential target of miR-224. miR-224 knockdown significantly increased CASP9 transcript and protein levels. Furthermore, luciferase reporter assays confirmed a direct interaction of miR-224 with CASP9. Our findings have demonstrated that the miR-224/CASP9 axis plays an important role in TNBC progression, providing evidence in support of a promising therapeutic strategy for this disease.