Figure 2: In vitro effects of the downregulation of miR-224. (a) AntagomiR-224 was transfected into MDA-MB-231 cells, and miR-224 expression was measured at 24, 48, and 72 hours posttransfection; . (b) AntimiR-224 significantly reduced MDA-MB-231 cell proliferation at 48 and 72 hours posttransfection, compared to the scramble control antimiR (40 μM), using the MTS assay; . (c) Representative images and quantification depicting the reduction of migratory ability (top) and invasion (bottom) of MDA-MB-231 cells that were transfected with 40 μM of antimiR-224 compared to the negative control antimiR. All data represent the from 3 independent experiments; .