Thyroxine Alleviates Energy Failure, Prevents Myocardial Cell Apoptosis, and Protects against Doxorubicin-Induced Cardiac Injury and Cardiac Dysfunction via the LKB1/AMPK/mTOR Axis in Mice

Figure 6

Effect of LKB1 KO on cardiac injury, energy failure, and myocardial cell apoptosis. (a) Heart sections were stained with HE, and the numbers of vacuolated cardiomyocytes in the four groups were quantified (200x). (b) Heart LDH and CK-MB levels were detected for each group. (c) Representative images and quantification of AMPK/mTOR axis phosphorylation. (d) Bcl2, Bax, and cleaved caspase-3 expression in the LV of each group. (e) Mitochondrial complex I, III, and IV activation was detected. (f) The cross-sectional area of the mitochondria in each group and more than 50 mitochondria were measured (5000x). (g) Representative images of TUNEL staining and the quantitative results for each group (200x); arrows indicate TUNEL-positive cells. in each group. vs. DOX+thyroxine group.

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