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Disease Markers
Volume 2019, Article ID 7945429, 7 pages
https://doi.org/10.1155/2019/7945429
Research Article

A Common Variant of ASAP1 Is Associated with Tuberculosis Susceptibility in the Han Chinese Population

1Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China
2School of Public Health, Fudan University, Shanghai, China
3Key Lab of Health Technology Assessment, National Health Commission of the People’s Republic of China, Fudan University, Shanghai, China
4Department of Public Health Sciences (Global Health/IHCAR), Karolinska Institutet, Stockholm, Sweden

Correspondence should be addressed to Biao Xu; nc.ude.umhs@uxb

Received 25 January 2019; Accepted 2 April 2019; Published 8 April 2019

Academic Editor: Hubertus Himmerich

Copyright © 2019 Cheng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. ASAP1 (also known as AMAP1 or DDEF1) encodes an Arf GTPase-activating protein (Arf GAP), a multifunctional scaffold protein that induces hydrolysis of GTP bound to the ADP-ribosylation factor (Arf) family GTP-binding proteins. Reduction of ASAP1 expression in vitro was related to suppression of cell migration and invasiveness. The genetic variant rs4733781 of the ASAP1 gene was revealed as a significant locus for tuberculosis (TB) susceptibility, but the results still need to be validated. Methods. Blood samples from a total of 1914 active TB and healthy controls (HC) were collected to evaluate rs4733781 and the risk of TB. Meanwhile, a total of 48 noninfected HC, latent TB-infected (LTBI) controls, and active TB were collected to assay ASAP1 expression difference among the three groups. The QuantiFERON-TB Gold In-Tube was adopted to identify noninfected HC and LTBI. Results. The genetic variant of rs4733781 was found to be significantly associated with TB, and the A allele of rs4733781 (C>A) was 0.38 and 0.43 among TB cases and HC, respectively (). Meanwhile, the peripheral blood monocyte RNA fold changes for the ASAP1 gene among the 16 HC, 16 LTBI, and 16 active TB were , , and (, ), respectively, and the expression of ASAP1 was increased by 2.06-fold () and 9.30-fold () for LTBI and active TB, when compared to the HC. Conclusions. Our data indicated that the A allele of rs4733781 for the ASAP1 gene was in association with a decreased risk of TB. But not only that, the overexpression of the ASAP1 gene among LTBI and TB was related to the progression of TB, which further implies that the expression of ASAP1 would be a potential biomarker for LTBI and TB diagnoses.