Long-Chain Fatty Acids and Inflammatory Markers Coaccumulate in the Skeletal Muscle of Sarcopenic Old Rats

Figure 6

(a) Resident immune cells in the quadriceps muscles of 21-month-old SD rats. The diagram shows the number of resident immune cells determined by direct microscopy of 10 randomly selected microscopic fields of Masson’s trichrome-stained muscle sections of all study animals. Bars indicate the from 12 CD-fed and 6 HFD-fed males (hatched and solid gray bars), as well as 5 CD-fed and 6 HFD-fed females (dotted and solid black bars, respectively). Statistical significance was determined using two-way ANOVA and is denoted by for . (b) Chemokine expression in the quadriceps muscles of 21-month-old SD rats. The diagram shows the concentration of each indicated chemokine (-axis) in 1 ng total tissue extract from 21-month-old rats as the of 12 CD-fed and 6 HFD-fed males (hatched and solid gray bars, respectively), as well as 5 CD-fed and 6 HFD-fed females (dotted and solid black bars, respectively). Statistical significance was determined using two-way ANOVA and is denoted by for and for .

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