Disease Markers / 2020 / Article / Fig 5

Research Article

6,12-Diphenyl-3, 9-diazatetraasterane-1, 5, 7, 11-tetracarboxylate Inhibits Proliferation, Migration and Promotes Apoptosis in Ovarian Cancer Cells

Figure 5

The effect of DDTC on expression of cell cycle regulators. The dosage of DDTC at 0.1 μM, 1 μM, and 10 μM in SKOV3 and A2780 cells. (a, b) Western blot assay showed that DDTC upregulated the expression of Chk1 protein level and downregulated the expression of Cdc25a and CDK1protein levels in SKOV3 cells. (c, d) Western blot assay showed that DDTC upregulated the expression of Chk1 protein level and downregulated the expression of Cdc25a and CDK1protein levels in A2780 cells. (e) The mRNA level of Chk1 was increased and level of Cdc25a and CDK1 were decreased in SKOV3 cells by Q-PCR. (f) The mRNA level of Chk1 was increased and level of Cdc25a and CDK1 were decreased in A2780 cells by Q-PCR. ; vs. the control group.
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