Strong-LAMP Assay Based on a Strongyloides spp.-Derived Partial Sequence in the 18S rRNA as Potential Biomarker for Strongyloidiasis Diagnosis in Human Urine Samples

Table 2

Reaction mixture (a) and amplification conditions used in TD-PCR using F3 and B3 external primers (b). Taq polymerase, buffer, MgCl₂, and dNTPs were supplied by Intron.

(a)

Components	Volume (μL)
H2O	17.1
10x buffer	2.5
MgCl2 (25 mM)	1.5
dNTPs (2.5 mM)	0.5
F3 (pmol)	0.5
B3 (pmol)	0.5
Taq polymerase (2 U)	0.4
Template DNA	2
Total	25

(b)

Temperature (°C)	Time (sec)	Cycle
94	60	X 1
94	20	X 2
57-52	20
72	30
94	60	X 15
51	20
72	30
72	60	X 1