Strong-LAMP Assay Based on a Strongyloides spp.-Derived Partial Sequence in the 18S rRNA as Potential Biomarker for Strongyloidiasis Diagnosis in Human Urine Samples

Table 3

Reaction mixture used in Strong-LAMP assays. Bst polymerase, buffer, and MgSO₄ were supplied by New England Biolabs, betaine by SIGMA, and the dNTPs by Intron.

Component	Volume (μL)
H2O	7.7
Betaine (1 M)	5
MgSO4	1.5
dNTPs (2.5 mM)	3.5
10x buffer	2.5
FIP (40 pmol/μL)	0.4
BIP (40 pmol/μL)	0.4
F3 (5 pmol/μL)	0.5
B3 (5 pmol/μL)	0.5
Bst polymerase 2.0	1
DNA	2
Total	25