Evaluation of a Novel Missense Mutation in ABCB4 Gene Causing Progressive Familial Intrahepatic Cholestasis Type 3

Figure 5

Effect of MG132 and bafilomycin A1 on MDR3 expression. MDR3 expression was examined in HEK-293T cells after transfection with ABCB4 wild-type and mutant plasmids. Western blot was performed after 24 hr treatment with MG132 (a) or bafilomycin A1 (b). Data shown represent mean of three independent experiments and analyzed by ANOVA followed by Tukey’s Multiple Comparison Test.

(a)

(b)