Research Article
Evaluation of a Novel Missense Mutation in ABCB4 Gene Causing Progressive Familial Intrahepatic Cholestasis Type 3
Figure 6
Effect of cyclosporin A and reduced temperature on ABCB4-WT and mutants. (a) HEK-293T cells expressing ABCB4-WT and mutants were treated with 10 μmol/L cyclosporin A for 24 hours. (b) HEK-293T cells were grown at 37°C for 24 hr and shifted to 30°C after transfection. Cells were processed for electrophoresis and immunoblotting with the anti-MDR3 P3II-26 antibody.
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