Molecular Markers for Detecting Schistosoma Species by Loop-Mediated Isothermal Amplification

Figure 6

Sensitivity assessment of LAMP for amplifying Schistosoma ITS-1 sequence using DNA from different schistosome species. (a) Detection limit for S. mansoni (1 pg). (b) Detection limit for S. haematobium (0.1 pg). (c) Detection limit for S. intercalatum (1 pg). (d) Detection limit for S. bovis (10 pg). Lanes Sm, Sh, Si, and Sb mean S. mansoni, S. haematobium, S. intercalatum, and S. bovis DNAs, respectively. Lanes 10^-1-10^-9: 10-fold serial dilutions of DNA. Lanes N: negative controls (no DNA template).

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