A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease

Table 2

Similarity degree among TriTrypDB sequences.

Target	Identity	value	Strain	Notes
Nuclear satellite DNA	100%		CL Brener/Tulahuen c12	Most abundant repetitive sequence in T. cruzi genome (up to 9%)
Putative flagellar calcium-binding protein	99%	0.0	CL Brener/JRc14/Tulahuen c12/Dm28c/Marinkellei	Sequence present in a single copy. Low sensitivity
E13 repeated element	97%	0.0	Tulahuen c12/CL Brener/Dm28c/Esmeraldo	Abundant repetitive sequence in T. cruzi genome (up to 7%)
Variable region of kinetoplast minicircles	100%		CL Brener/Tulahuen c12/JRc14/Esmeraldo	Variable region with low specificity for LAMP primer design
24S ribosomal RNA alpha-subunit	97%		JRc14/SylvioX10/Dm28c/Tulahuen c12/Marinkellei/CL Brener/Esmeraldo	Small size for LAMP design and very conserved in trypanosomatids
Ribosomal intergenic spacer	95%		JRc14/SylvioX10/Dm28c/Tulahuen c12/Esmeraldo/Brener	Scarcely used for T. cruzi detection
Transposon-like E22 repeated element	98%	0.0	CL Brener/Tulahuen c12/Esmeraldo/Dm28c	Scarcely used for T. cruzi detection
Subtelomeric conserved junction	96%	0.0	Tulahuen c12/JRc14/CL Brener/Esmeraldo/Dm28c	Scarcely used for T. cruzi detection

Consult the bibliography shown in Table 1 for more details about sequences.