Research Article
A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease
Table 3
Sequences and characteristics of the primers used.
| | Primer | Len | Tm | 3dG | GC rate (%) | Sequence (5 to 3) |
| | F3 | 18 | 60.8 | -1 | 50 | AACTATCCGCTGCTTGGA | | B3 | 18 | 60.2 | -0.4 | 50 | AAGAGCTCGCGAAATTCC | | FIP (F1c-F2) | 41 | | | | CCCACCATTCACAATCGGAAACCACTCGGCTGATCGTTTT | | BIP (B1c-B2) | 41 | | | | AGTCAGAGGCACTCTCTGTCAACCAAGCAGCGGATAGTTC | | F2 | 19 | 60.3 | -0.7 | 50 | CACTCGGCTGATCGTTTT | | F1c | 20 | 65.1 | 0.1 | 55.6 | CCCACCATTCACAATCGGAAAC | | B2 | 18 | 65.1 | -0.9 | 50 | CCAAGCAGCGGATAGTTC | | B1c | 22 | 72.5 | -0.9 | 50 | AGTCAGAGGCACTCTCTGTCAA | | LF | 19 | 60.1 | 0.1 | 50 | TTGGACCACAACGTGTGAT | | LB | 20 | 64.4 | -0.4 | 50 | TTCACACACTGGACACCAAA |
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