Evaluation of Altered Glutamatergic Activity in a Piglet Model of Hypoxic-Ischemic Brain Damage Using ¹H-MRS

Figure 4

AMPAR subunit GluR2 protein levels in the basal ganglia before (control) and after HI injury (400x magnification). Scale . (a) High GluR2 protein levels in the basal ganglia in the control group. (b) At 72 h after HI injury, the GluR2 protein level was markedly reduced. (c) Changes in the average OD of GluR2 protein level visualized with IHC staining. GluR2 protein level declined in piglets subjected to HI injury compared with the control. (d) GluR2 protein level was negatively correlated with the severity of pathological lesions; i.e., the more severe the brain injury, the more obvious the downregulation of GluR2 protein level. AMPAR: α-amino-3-hydroxy-5-methyl-4-isoxazole-proprionic acid receptor; IHC: immunohistochemical; OD: optical density. Error bars represent the standard deviation values (/group). compared with the control group. The Spearman rank correlation coefficient was presented as .

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